antibax (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
antibax
Antibax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antibax/Bax+Rabbit+mAb/pm41366047-239-41-43
Average 96 stars, based on 1880 article reviews
Antibax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+antibax/Bax+Rabbit+mAb/pm41366047-239-41-43
Average 96 stars, based on 1880 article reviews
antibax - by Bioz Stars,
2026-09
96/100 stars
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Protein Concentration:Article Title: P2X7 Receptor of Microglia Mediates Neuropathic pain by Regulating Autophagy After Chronic-Constriction Injury Article Snippet: .. Lysates were centrifuged at 12000 rpm for 15 min at 4°C, and protein concentration was determined by a bicinchoninic acid (BCA) Protein Assay Kit (Beyotime Institute of Page 5/23 Biotechnology, Shanghai, China).Samples with equal amount of protein were separated by 9%-12%PAGE (40g total protein per lane) and transferred onto polyvinylidene di uoride membranes (Millipore, USA), followed by blockage with 5% skim milk for 2h and incubation overnight with the following primary polyclonal antibodies: rabbit anti-P2X7R (1:250; Alomone, Israel),rabbit anti-LC3B (1:1000, Cell Signaling Technology, Beverly, MA, United States), rabbit polyclonal anti Beclin 1 (1:1000, MBL International Corporation, Nagoya, Japan), rabbit anti-Bcl-2 (1:1000; 2876, Cell Signaling Technology, USA), Bicinchoninic Acid Protein Assay:Article Title: P2X7 Receptor of Microglia Mediates Neuropathic pain by Regulating Autophagy After Chronic-Constriction Injury Article Snippet: .. Lysates were centrifuged at 12000 rpm for 15 min at 4°C, and protein concentration was determined by a bicinchoninic acid (BCA) Protein Assay Kit (Beyotime Institute of Page 5/23 Biotechnology, Shanghai, China).Samples with equal amount of protein were separated by 9%-12%PAGE (40g total protein per lane) and transferred onto polyvinylidene di uoride membranes (Millipore, USA), followed by blockage with 5% skim milk for 2h and incubation overnight with the following primary polyclonal antibodies: rabbit anti-P2X7R (1:250; Alomone, Israel),rabbit anti-LC3B (1:1000, Cell Signaling Technology, Beverly, MA, United States), rabbit polyclonal anti Beclin 1 (1:1000, MBL International Corporation, Nagoya, Japan), rabbit anti-Bcl-2 (1:1000; 2876, Cell Signaling Technology, USA), Incubation:Article Title: P2X7 Receptor of Microglia Mediates Neuropathic pain by Regulating Autophagy After Chronic-Constriction Injury Article Snippet: .. Lysates were centrifuged at 12000 rpm for 15 min at 4°C, and protein concentration was determined by a bicinchoninic acid (BCA) Protein Assay Kit (Beyotime Institute of Page 5/23 Biotechnology, Shanghai, China).Samples with equal amount of protein were separated by 9%-12%PAGE (40g total protein per lane) and transferred onto polyvinylidene di uoride membranes (Millipore, USA), followed by blockage with 5% skim milk for 2h and incubation overnight with the following primary polyclonal antibodies: rabbit anti-P2X7R (1:250; Alomone, Israel),rabbit anti-LC3B (1:1000, Cell Signaling Technology, Beverly, MA, United States), rabbit polyclonal anti Beclin 1 (1:1000, MBL International Corporation, Nagoya, Japan), rabbit anti-Bcl-2 (1:1000; 2876, Cell Signaling Technology, USA), Article Title: Over-expression of miR-3584-5p Represses Nav1.8 Channel Aggravating Neuropathic Pain caused by Chronic Constriction Injury. Article Snippet: Nav1.8, a tetrodotoxin-resistant voltage-gated sodium channels (VGSCs) subtype encoded by SCN10A, which plays an important role in the production and transmission of peripheral neuropathic pain signals.. Studies have shown that VGSCs may be key targets of MicroRNAs (miRNAs) in the regulation of neuropathic pain.. In our study, bioinformatics analysis showed that the targeting relationship between miR-3584-5p and Nav1.8 was the most closely. Western Blot:Article Title: E3 ubiquitin ligase PARK2, an inhibitor of melanoma cell growth, is repressed by the oncogenic ERK1/2-ELK1 transcriptional axis Article Snippet: .. Antibodies used for Western blot were: mouse anti-PARK2 (#4211), rabbit anti-pERK1/2 (#9101), mouse anti-BCL-2 (#15071), rabbit anti-BCL-XL (#2764), Article Title: Dysregulation of the p53 pathway provides a therapeutic target in aggressive pediatric sarcomas with stem-like traits. Article Snippet: 1 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno 625 00, Czech Republic 2 International Clinical Research Center, St. Anne’s University Hospital, Brno 656 91, Czech Republic 3 1st Department of Pathology, St. Anne’s University Hospital and Faculty of Medicine, Masaryk University, Brno, Czech Republic 4 Department of Pediatric Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno 613 00, Czech Republic 5 Central European Institute of Technology (CEITEC), Masaryk University, Brno 625 00, Czech Republic 6 Department of Internal Medicine, Hematology and Oncology, and Institute of Medical Genetics and Genomics, University Hospital Brno and Faculty of Medicine, Masaryk University, Brno 625 00, Czech Republic Abstract Purpose Pediatric sarcomas are bone and soft tissue tumors that often exhibit high metastatic potential and refractory stemlike phenotypes, resulting in poor outcomes.. Aggressive sarcomas frequently harbor a disrupted p53 pathway.. However, whether pediatric sarcoma stemness is associated with abrogated p53 function and might be attenuated via p53 reactivation remains unclear. |